Primary Outcome Measures
- Procurement of primary and metastatic tissue of thoracic malignancies shortly after death in order to investigate tumor heterogeneity- both intratumor and between paired primary and metastatic site, using integrated genomic and proteomic analysi… [ Time Frame: Death ] [ Designated as safety issue: No ]
Detailed Description
Background
- Despite being the leading cause of cancer-related death worldwide, there is only limited knowledge of tumor heterogeneity in lung cancer. There is also limited knowledge of tumor heterogeneity of other less common thoracic malignancies, such as thymic epithelial tumors and mesothelioma.
- Tumor heterogeneity can be evaluated in a comprehensive manner by deep sequencing and global analysis of genomic and proteomic alterations of simultaneous core biopsies from several areas of the primary tumor and metastases and correlation with clinical outcome. However such studies are not feasible in a clinical setting.
- Tissue procurement by rapid autopsies provides an effective way for such an investigation.
Hypothesis
- Clonal evolution and selection of tumor cells can be assessed by examining genomic and proteomic alterations of tumor samples obtained from multiple sites of primary and metastatic sites.
Design
- Twelve patients each of NSCLC, SCLC, thymic epithelial tumors, and mesothelioma, and six each of ESCCs and pNETs will be autopsied in this pilot study.
- Patients will be admitted for inpatient hospice when an investigator estimates a survival of less than 2 weeks.
- Upon expiration, rapid autopsy will be performed and tissue obtained from primary tumor site if still identifiable, and multiple metastatic sites to assess tumor heterogeneity using deep sequencing and global genomic and proteomic analyses.
- Archival tissue from patients, if available, will be used to evaluate these changes from several stages of tumor progression.
Eligibility
- Inclusion Criteria
- Patients must have histologically or cytologically confirmed metastatic NSCLC, SCLC, EPCC, pNET, thymic epithelial tumor (thymoma, thymic carcinoma) or mesothelioma confirmed by the NCI Laboratory of Pathology.
- Age greater than 18 years.
- Life expectancy less than or equal to 3 months.
- Patients or their previously designated Durable Power of Attorney (DPA) (if the patient is deemed by the treating physician to be impaired or questionably impaired in such a way that the ability of the patient to give informed consent is questionable) must sign an informed consent indicating that they are aware of the investigational nature of this study.
- Patients or their previously designated DPA and their legal next of kin must agree to a Do Not Resuscitate (DNR) order and agree to Consent for Autopsy as part of the end of life care plan.
- This study was designed to include women and minorities, but was not designed to measure differences of intervention effects. Males and females will be recruited with no preference to gender. No exclusion to this study will be based on race. Minorities will actively be recruited to participate.
- Exclusion Criteria
- Women known to be pregnant (known positive pregnancy test, although such testing is not required for enrollment) are excluded.
- Known HIV-positive patients will be excluded (although HIV testing is not required for enrollment) because of the potential for contamination of tissue.
- Age greater than 18 years.
